![]() #Western blot vs southern blot codeWhen a DNA sequence is the foundation or code for a protein molecule, the particular DNA molecule of interest can be blotted using Southern Blotting technique. Subtypes of blotting such as northern, western & southern depend upon the target molecule that is being sought. The other blotting techniques emerged from this method have been termed as Northern (for RNA), Western (for proteins), Eastern (for post-translational protein modifications) and South-western (for DNA-protein interactions) blotting. Southern blotting was introduced by Edwin Southern in 1975 as a method to detect specific sequences of DNA in DNA samples. After the molecules are transferred to the membrane their position does not change. The hence processed molecules are required to be hard-pressed against a suitable membrane which will in turn transfer the molecules from the gel onto a suitable membrane (nylon, nitrocellulose or PVDF) via capillary action. Blotting is performed by allowing a mixture of molecules of interest pass through a block of gel which separates the molecules based on their molecular sizes. In the case of a cell, these molecules are present altogether and hence with the help of blotting scientists are able to recognise a specific molecule out of all others. ![]() Molecules such as DNA, RNA and proteins are subjected to biochemistry analysis which are separated using blotting techniques. Protein synthesis involves expression of a DNA segment which gets converted to mRNA to produce the respective protein. The blotting technique is a tool used in the identification of biomolecules such ad DNA, mRNA and protein during different stages of gene expression. It uses hybridization techniques for the identification of the specific nucleic acids and genes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. ![]()
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